FAQ: How many cells should I seed into cell culture plates ...

Following general cell culture guidelines, do not seed cells to exceed average yields per well at 100% confluence (refer to manufactories&#; guidelines, the table below is modified from Corning Inc. as an example). Keep in mind, cells might still be dividing after seeding. Make sure the cell number at harvest is within the lysis capacity. In addition, an initial test may help determine the optimal range of cell inputs for your specific cell lines or amplicons.

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Cell culture plates

Average Yields (100% confluence)

6-wells

9.5 x 105

12-wells

3.8 x 105

24-wells

1.9 x 105

48-wells

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0.95 x 105

96-wells

3.2 x 104

384-wells

5.6 x 103